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Indian Institute of Science Education and Research Bhopal

BIO 307: Biology Laboratory-I (3)

• Isolation of total RNA from Plant Tissues.
• Isolation of Plant Chloroplast or mitochondria (any one)
• Protoplast preparation/ visualization of stomata
• Coleoptile bending experiment
• Fractionation of cellular organelles using centrifugation and validation by immunoblotting.
• Enzyme kinetics (with or without inhibition) for alkaline phosphatase.
• Purification of proteins using affinity chromatography
• Detection of proteins by CBBR and silver staining
• Size exclusion chromatography for analysis of proteins and nucleic acids
• Introduction to animal cell culture
• Immunofluorescence

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