Biological Sciences
BIO 307: Biology Laboratory-I (3)
- Isolation of total RNA from Plant Tissues.
- Isolation of Plant Chloroplast or mitochondria (any one)
- Protoplast preparation/ visualization of stomata
- Coleoptile bending experiment
- Fractionation of cellular organelles using centrifugation and validation by immunoblotting.
- Enzyme kinetics (with or without inhibition) for alkaline phosphatase.
- Purification of proteins using affinity chromatography
- Detection of proteins by CBBR and silver staining
- Size exclusion chromatography for analysis of proteins and nucleic acids
- Introduction to animal cell culture
- Immunofluorescence
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