Office of Academic Affairs
Indian Institute of Science Education and Research Bhopal
Biological Sciences
BIO 308: Biology Laboratory-II (3)
- Estimation of Hemoglobin using hemometer.
- Estimation of Sugar in blood/urine by glucometer.
- Analyzing the components of blood.
- In vitro transcription.
- In vitro translation.
- Gene amplification from bacterial genome and cloning into commercial vectors; verification of positive clones.
- Protein expression screening in BL21 strains and one-step purification from whole cell lysates.
- Analysis of chromatin structure by Micococcal nuclease.
- Analysis of histones by 2-Dimensional gel electrophoresis.
- Analysis of protein expression upon DNA damage.
- Yeast transformation.
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