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Indian Institute of Science Education and Research Bhopal

BIO 408: Bioinstrumentation (4)

Spectroscopy (UV/Vis spectroscopy, Fluorescence spectroscopy, CD spectroscopy); Principle of centrifugation (Density gradient centrifugation; Ultracentrifugation; Analytical centrifugation); Microscopy; (Bright field and dark field microscopy; Fluorescence microscopy; Confocal microscopy; Electron microscopy); Sequencing of protein and nucleic acid; Next generation sequencing; Realtime QPCR; Chromatography (Ion exchange, Gel filtration, Affinity, Thin-layer); Radioisotope technology. SPR, mass spectrometry, NMR and XRD instrumentation

Suggested Books:

• Analytical ultracentrifugation for the study of protein association and assembly, Geoffrey J Howlett, Allen P Minton, Germán RivasCurrent Opinion in Chemical Biology Volume 10, Issue 5, October 2006, Pages 430–436
• Analytical Ultracentrifugation: Sedimentation Velocity and Sedimentation Equilibrium. James L. Cole, Jeffrey W. Lary, Thomas Moody, and Thomas M. Laue
• Methods Cell Biol. 2008; 84: 143–179
• Molecular Cloning by Sambrook and Russel Chapter 12
• How to study proteins by circular dichroism Biochim. Biophys. Acta (2005) 1751: 119
• Principles of Fluorescence Spectroscopy by Lakowicz
• Confocal Microscopy for Biologists by Alan R. Hibbs
• Handbook of Biological Confocal Microscopy by James Pawley

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