Office of Academic Affairs
Indian Institute of Science Education and Research Bhopal
Biological Sciences
BIO 405: Biology Laboratory-III (3)
- Immunization of mouse/Rabbit by defined antigen such as OVA
- Estimation of antibody by ELISA
- Preparation peritoneal exudates cells (PECs) from mouse by thioglycollate injection
- Isolation of PECs
- Cell counting
- Culturing and stimulation of PECs with LPS
- Estimation of cytokine by ELISA
- Western blotting experiment
- Modeling protein and DNA secondary structures and tertiary folds (group experiment)
- Protein/DNA secondary structure estimation
- Thermodynamic estimation of protein/DNA stability
- Protein degradation under various conditions.
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