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Indian Institute of Science Education and Research Bhopal

Biological Sciences

BIO 304:  Molecular Biology (3)

DNA replication: Unit of replication, Enzymes involved, origin of replication and replication fork, Fidelity of replication, extrachromosomal replicons; DNA damage and repair- mechanisms, homologous and site-specific recombination; RNA Synthesis: transcription factors and machinery; initiation complex formation, activators and repressors of transcription, RNA polymerases, capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, different types RNA: structure and functions, RNA transport; Protein synthesis: Ribosome, formation of initiation complex and regulation of initiation factors, elongation and elongation factors, termination, concept of genetic code, translation: aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and proof-reading, inhibitors of translation, post- translational modification of proteins; Protein structure-function correlation: DNA recognition, enzyme catalysis, membrane proteins, proteins of the immune system, viruses; Protein degradation; Protein engineering and design; Techniques for study of protein structures; Regulation of gene expression: Gene expression control at transcription and translational level; Chromatin and gene expression; Gene silencing. siRNA and microRNA; Restriction endonucleases, Recombinant DNA technology and applications: Cloning, plasmid vectors (shuttle and expression vector, RFLP, electrophoresis, heterologous protein expression and promoters). Hybridization processes (Northern, southern and Western)

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