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Indian Institute of Science Education and Research Bhopal

BIO 203: General Biology Laboratory III (1)

• Plasmid isolation
• Competent cell preparation
• Transformation and calculation of the efficiency
• Nucleic acid estimation
• Polymerase Chain reaction
• Agarose Gel electrophoresis
• Poly Acrylamide Gel Eelctrophoresis (PAGE)
• Plasmid restriction mapping
• Genomic DNA isolation

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