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Indian Institute of Science Education and Research Bhopal

CHM 629: Advanced Molecular Spectroscopy (4)

Prerequisites: CHM 322/642, CHM 422/622 or their equivalent

Basic Concepts: Einstein’s coefficients and their relation with transition moment integral, time-dependent perturbation theory, various kinds of transitions and selection rules, notations of energy levels and electronic transitions

Fluorescence: Introduction, Jablonskii diagram, kinetic parameters, relationship between lifetime and Einstein’s coefficients (Strickler and Berg equation) and its limitations, fluorescence quantum yield, Stokes’ shift, analysis of a fluorescence spectrum, effect of solvent on fluorescence, (general effects and specific effects), Lippert equation and its applications, fluorescence excitation spectra, time scales of molecular processes, fluorescence quenching, different types of mechanisms associated with fluorescence quenching and their applications, fluorescence anisotropy and its applications

Radiationless processes: Mechanism of internal conversion and inter-system crossing, effect of temperature on radiationless processes

Phosphorescence: Kinetic parameters, origin of triplet state and its formation, different methods of triplet-triplet absorption, factors affecting the rate of phosphorescence

Resonance Energy Transfer: Different mechanisms of energy transfer (Forster and Dexter), selection rules for energy transfer, non-vertical energy transfer, FRET and its applications, typical examples and choice of dyes.

Spectrophotometry and Fluorometry: Principles and instrumentation, choice of light sources and detectors, monochromators, optical filters and choice of filters, fluorophores and dyes used in spectroscopy, intrinsic and extrinsic fluorophores, protein labeling

Lasers and its applications: Principles of lasers, multi-photon ionization processes in molecules, dynamics of reactions in liquids, spectroscopy of single molecules, concept of time-resolved spectroscopy, Confocal microscopy, FCS and FLIM.

Suggested Readings :

• Atkins, P. W., and de, Paula, J., Physical Chemistry, 8th Edition, Oxford Press, 2008.
• Levine, I., Physical Chemistry, McGraw-Hill, 2008.
• Lakowicz, J. R., Principles of Fluorescence Spectroscopy, Plenum Press, 2003.
• Dogra, S. K., and Randhawa, H. S.; Atom, Moleculeand Spectrum, New Age International Pvt. Ltd., 2011.
• Turro, N. J., Ramamurthy, V., and Scaiano, J. C., Principles of Molecular Photochemistry: An Introduction, University Science Books, 2009.
• Selvin, P. R., and Ha, T., Single Molecule Techniques: A Laboratory Manual, Cold Spring HarborLaboratory Press, 2008.

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