BIO 613S: Immunotechnology (2)
Basic Antigen-Antibody Interaction based techniques - Agglutination, Precipitation, Flocculation, ELISA, ELIPSOT, RIA, Immunoblotting, Immunofluorescence, Polyclonal and Monoclonal antibody, FACS and MACS, Ferritin-Antibody Conjugate System
Classical Serological Techniques - Cellular Antigen, Agglutination-Haemagglutination Assay, Haemagglutinin Inhibition and Blood grouping, Lysis-Bacteriolysis and Haemolysis, Soluble Antigen-Precipitation, Immunodiffusion and Immunoelectrophoresis (1D, 2D and Radial)
Classical Serological cell based Techniques - Complement Fixation, Coomb’s test, HLA typing, Tetramer Assay, Mixed Lymphocyte Reaction
Basic nucleic acid based Techniques - PCR Based Methods- Real-Time and Reverse Transcriptase PCR, Chromatin Immunoprecipitation.
Suggested Books:
- Essential immunology by Ivan M. Roitt
- Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications by R. Ian Freshney.
- Immunobiology by Janeway
- Latest research papers.