Office of Academic Affairs
Indian Institute of Science Education and Research Bhopal

Biological Sciences

BIO 611S: Cell Science and Technology (2)

Basics of Cell Culture - Cell culture- primary culture and secondary culture, confluence, different morphologies of cell culture. Types and use of monolayer, suspension culture, different uses based on cell-types. How in vitro experiments are different from in vivo, special requirements for cells for culture and its importance in immunological studies. Cryogenic Preservation, Storage and Recovery Different Biosafety Levels Labs/facility, Need for biosafety Labs/facility. Contamination in animal cell culture

Handling and Manipulating Cell Cultures - Passaging techniques by trypsin, scraping, EDTA, passaging of suspension cultures. Introduction of foreign material into cells through different transfection and electroporation techniques.

Cloning of gene and its Identification - Characteristics of vector and foreign DNA and uses based up on the size of DNA fragment being cloned. Mammalian expression vectors and its application. Different strategies for identification of clone-insertional inactivation, blue-white colony screening Site-directed mutagenesis

Techniques associated with Study of Gene - Studying location of a gene, Fluorescent In-Situ Hybridization, Genome In-Situ Hybridization, Studying Location and Dynamics of Gene Expression- Bioluminescence [luciferase assay and in vivo imaging], Microarray Technology

Cell Therapy as Regenerative Medicine - Organ Culture, Stem cell therapy, Cases of cardiovascular diseases and diabetes, Generating blood cells for transfusion

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